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Transfected Stable Cell Lines

CD63 Knockout 293T Cell Line

  • For research use only

Cat No.

ABC-KH024Y
Product Type

Knockout Stable Cell Line
Cell Type

Epithelial
Species

Human
Host Cell

293T
Source Organ

Kidney
Disease

Normal
Storage

Liquid Nitrogen

CD63 Knockout 293T Cell Line by AcceGen enables essential research on exosome biogenesis, tetraspanin networks, and immune synapse formation.

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  • CD63 Knockout 293T Cell Line
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Home | Transfected Stable Cell Lines | CD63 Knockout 293T Cell Line

Description

CD63 Knockout 293T Cell Line is generated from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the Cluster of Differentiation 63 (CD63) gene, a tetraspanin family protein that regulates exosome biogenesis, intracellular trafficking, and membrane protein organization. This knockout model exhibits impaired exosomal marker sorting and altered endolysosomal trafficking while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line serves as an essential tool for studying extracellular vesicle biology, viral entry mechanisms (particularly HIV-1 infection), and cancer metastasis, with special utility in research involving exosome-based diagnostics and therapeutics. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and flow cytometry analysis demonstrating loss of CD63 surface expression. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.

Species

Human
Cat.No

ABC-KH024Y
Product Category

Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Epithelial
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Kidney
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Knockout Stable Cell Line
Host Cell

293T
Gene Info

CD63
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • The CD63 Knockout 293T Cell Line is a specialized model for studying extracellular vesicle biogenesis and cellular trafficking pathways. This engineered system enables investigation of tetraspanin CD63’s roles in exosome formation, viral entry mechanisms, and membrane protein organization. Researchers utilize this knockout line to examine disrupted endosomal sorting and altered extracellular vesicle cargo composition. The cell line provides a controlled platform for analyzing exosome-mediated intercellular communication and developing targeted therapeutic strategies for infectious diseases and cancer.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

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High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
Tailed to your research

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