CHD1 Knockout 293T Cell Line

Description

CHD1 Knockout 293T Cell Line is engineered from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the Chromodomain Helicase DNA Binding Protein 1 (CHD1) gene, an ATP-dependent chromatin remodeler essential for nucleosome organization, transcriptional regulation, and genome stability. This knockout model exhibits impaired chromatin remodeling and transcriptional dysregulation while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line provides a powerful tool for studying epigenetic regulation, cancer development (particularly prostate cancer), and stem cell pluripotency, with special applications in research involving androgen receptor signaling and DNA damage response. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and chromatin accessibility assays (ATAC-seq). The cell line has undergone rigorous quality control and is certified free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.