DMS 454

DMS-454 is a human small cell lung carcinoma (SCLC) cell line established from the lung tissue of a Caucasian male patient during autopsy. It displays polygonal morphology with adherent growth characteristics and contains dense-core vesicles, secreting calcitonin, indicating neuroendocrine features common to SCLC. The cells can be cultured in Waymouth’s MB 752/1 medium supplemented with 2mM glutamine and 10% fetal bovine serum (FBS), or alternatively in RPMI 1640 with 10% FBS [1]. Routine subculture involves digestion with 0.25% trypsin or trypsin/EDTA and a 1:3 split ratio when cultures reach sub-confluence. Cytogenetic analysis has not been extensively reported, and specific gene mutations remain undefined. However, DMS-454 shares key biological behaviors with other SCLC lines, including likely tumorigenicity and high metastatic potential based on the aggressive nature of its tumor origin. It is suitable for studies on neuroendocrine tumor biology and calcitonin-secreting carcinomas.