HighQC™ Human IPSC From Fibroblast-Amyotrophic Lateral Sclerosis

Description

HighQC™ Human iPSC from Fibroblast – Amyotrophic Lateral Sclerosis (ALS) is an induced pluripotent stem cell (iPSC) line derived from human skin fibroblasts collected from a 50-year-old Caucasian male ALS patient. The cells exhibit the classic iPSC morphology of flat, round, tightly packed colonies. These cells were reprogrammed using a combination of transcription factors including OCT4, SOX2, KLF4, and c-MYC, delivered via a non-integrating Sendai viral vector system. They maintain pluripotency and express stemness markers such as OCT4, SOX2, and NANOG, as confirmed by immunocytochemistry. Karyotype analysis reveals both 46,XY and a minor 47,XY clone. No ALS-associated mutations such as in SOD1, TDP-43, or C9orf72 were detected. The iPSCs are non-tumorigenic . They can be directed into neural subtypes such as motor neurons and astrocytes, making them highly suitable for ALS disease modeling, drug screening, and neurotoxicity testing