Human Acute Myeloid Leukemia Peripheral Blood Mononuclear Cells (Newly Diagnosed)

Description

Human Acute Myeloid Leukemia Peripheral Blood Mononuclear Cells (Newly Diagnosed) are isolated from the peripheral blood of newly diagnosed acute myeloid leukemia (AML) patients. The mononuclear cell-rich PBMC layer is separated from diluted whole blood by gradient centrifugation. This cell line shows a heterogeneous cell population, including immature myeloid progenitor cells, monocytes and a small number of lymphocytes. Cytogenetic analysis of these cells commonly reveals chromosomal abnormalities such as t(8;21), inv(16), or monosomy 7, depending on the patient. In in vitro culture, this cell population shows the characteristics of coexistence of adherent growth and suspended growth, and some myeloid progenitor cells can differentiate into leukemic blasts with diverse morphologies. These cells typically express CD34, CD33, and HLA-DR, detectable via flow cytometry. They are tumorigenic and can initiate leukemic proliferation when engrafted into immunodeficient mice. In translational research settings, these samples are widely used for PBMC gene expression profiling to identify AML-related biomarkers, monitor disease progression, complement clinical blood analyses, and support studies of mononuclear cell involvement in human leukemia.