Human CD16-/CD66b+ Eosinophils-Negatively Selected

Description

Human CD16⁻/CD66b⁺ Eosinophils (Negatively Selected) are primary granulocytes isolated from peripheral blood through negative selection, resulting in an enriched population of eosinophils with depletion of other leukocyte subsets. These eosinophils are key effectors in immune defense mechanisms against parasitic helminths, bacterial and viral pathogens, and contribute to the pathophysiology of allergic inflammatory diseases such as asthma. Morphologically, they are characterized by bilobed nuclei and cytoplasmic granules containing cytotoxic proteins including eosinophil peroxidase (EPO) and major basic protein (MBP). CD66b (CEACAM8), a glycosylphosphatidylinositol (GPI)-anchored surface adhesion molecule highly expressed on eosinophils, mediates cell adhesion and activation during inflammatory responses. The cells are cryopreserved shortly after isolation at an early stage and are non-proliferative in culture, thus not suitable for passaging. To preserve functional integrity and viability, repeated freeze-thaw cycles should be strictly avoided.