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Primary Cells

Human CD16-/CD66b+ Eosinophils-Negatively Selected

  • BSL

    1

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Granulocytes gated by high side scatter were assessed for CD15 and CD16 to enumerate neutrophils (CD15+CD16+) and eosinophils (CD15+CD16-). Anticoagulant/Specification Type: Na Heparin
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Species

Human

Cat.No

ABC-TC4372

Quality Control

All cell lots test negative for HIV-1, HBV, HCV, mycoplasma, yeast, fungi, and other pathogens.

Product Category Primary Cells
Size/Quantity

1M

Cell Type

Eosinophil

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Disease

Normal

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Immune Cells

Key Features

– Backed by AcceGen advanced technology

– Cryopreserved for highest viability and plating efficiency

– Quality-guaranteed for accurate results

– Timely technical support for best experimental outcomes

Description

Human CD16-/CD66b+ Eosinophils-Negatively Selected are primary granulocytes isolated from peripheral blood, enriched for eosinophils and depleted of other cell types. These eosinophils play a critical role in immune defense against parasitic, bacterial, and viral infections, as well as in allergic inflammatory responses like asthma. They exhibit bilobed nuclei and cytotoxic granules containing proteins like eosinophil peroxidase and major basic protein (MBP). CD66b, a surface protein highly expressed on these eosinophils, regulates their adhesion and activation during immune responses. The cells are cryopreserved at early passage and cannot proliferate or be passaged. Repeated freeze-thaw cycles should be avoided to maintain their functionality and viability.

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Citation

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Application

  • Human CD16-/CD66b+ eosinophils serve as an accessible model for studying the biology and pathophysiology of eosinophils.. Eosinophils are implicated in a range of diseases, including asthma, eosinophilic gastrointestinal (GI) disorders, and hypereosinophilic syndrome. Further investigation targeting eosinophils could offer valuable insights into treatments for eosinophil-associated diseases..

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