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Transfected Stable Cell Lines

Human cKIT (V559D_D820G) BAF3 Cell Line

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Home | Transfected Stable Cell Lines | Overexpression Stable Cell Lines | Human cKIT (V559D_D820G) BAF3 Cell Line
Species

Human
Cat.No

ABC-X0381C
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Lymphocytes
Shipping Info

Dry Ice
Growth Conditions

37 °C, 5% CO2
Source Organ

Lymphatic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Overexpression Stable Cell Lines
Host Cell

BAF3

Description

Human cKIT (V559D_D820G) BAF3 Cell Line is a genetically engineered model derived from selected murine pro-B Ba/F3 parental cell line based on customers’ requirement. cKIT (V559D-D820G) BAF3 overexpression cell line is generated by stable integration of exogenous human cKIT gene containing V559D and D820G mutations into BAF3 host cells using our optimized transduction of lentiviral vectors. Overexpression clone is validated at gene level by qRT-PCR.
Target
The V559D and D820G mutations in cKIT enhance kinase activity and are implicated in oncogenesis and resistance to specific inhibitors. These mutations are clinically relevant in gastrointestinal stromal tumors and acute myeloid leukemia. AcceGen offers generation of stable overexpression cell lines targeting any gene of your interest. Polyclonal or monoclonal is optional based on customers’ research needs.

AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • For research use only

  • The cKIT (V559D-D820G) BAF3 Overexpression Cell Line serves as a preclinical model for dissecting dual-mutant cKIT function and evaluating next-generation inhibitors.

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