Human KRT14 Knockout A-431 Cell Line

Description

Human KRT14 Knockout A-431 Cell Line is generated from human epidermoid carcinoma A-431 cells through CRISPR/Cas9-mediated disruption of the Keratin 14 (KRT14) gene, a type I intermediate filament protein essential for epidermal integrity and mechanical stability. This knockout model exhibits compromised cytoskeletal architecture and reduced cell adhesion while maintaining A-431 cells’ characteristic epithelial morphology, though with increased fragility. The cell line serves as a valuable tool for studying skin blistering disorders (particularly epidermolysis bullosa simplex), epithelial stress responses, and tumor invasion mechanisms, with special utility in research involving mechanical stress signaling and epithelial-mesenchymal plasticity. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through triple verification (genomic PCR, Sanger sequencing, and immunofluorescence microscopy) demonstrating complete loss of KRT14 filament networks. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.