Human MMC treated Dermal Fibroblasts

Description

Human MMC Treated Dermal Fibroblasts are primary human dermal fibroblasts treated with mitomycin C (MMC), an agent that inhibits cell proliferation and induces apoptosis. Following primary culture, these cells are cryopreserved. MMC treatment results in altered gene expression, including downregulation of TGF-β1 and type I/III procollagen and modulation of bFGF mRNA levels. These changes are relevant in studies of wound healing and fibrotic diseases. MMC-treated fibroblasts are particularly useful in co-culture models or as feeder layers where proliferation inhibition is desired. In vitro, these cells exhibit limited proliferative capacity. Repeated freeze-thaw cycles should be avoided. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.