Human NBN Knockout A-431 Cell Line

Description

Human NBN Knockout A-431 Cell Line is generated from human epidermoid carcinoma A-431 cells through CRISPR/Cas9-mediated disruption of the Nibrin (NBN/NBS1) gene, a core component of the MRE11-RAD50-NBN (MRN) complex essential for DNA damage response and genomic stability. This knockout model exhibits defective DNA double-strand break repair and impaired ATM/ATR signaling while maintaining A-431 cells’ characteristic epithelial morphology. The cell line provides a critical tool for studying radiation sensitivity, cancer predisposition syndromes (particularly Nijmegen breakage syndrome), and chemotherapy resistance mechanisms, with special applications in research involving homologous recombination and telomere maintenance. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through multiplex analysis including genomic PCR, Sanger sequencing, and functional γ-H2AX foci formation assays following ionizing radiation. The cell line has undergone rigorous quality control and is certified free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.

Application

• FOR RESEARCH USE ONLY
  

• The Human NBN Knockout A-431 Cell Line is a specialized model for studying DNA damage response and genomic instability in epithelial cancers. This engineered system enables investigation of nibrin’s role in double-strand break repair and cell cycle checkpoint control. Researchers utilize this knockout line to examine defective homologous recombination and increased radiosensitivity in squamous cell carcinoma. The cell line provides a controlled platform for evaluating DNA repair-targeted therapies and synthetic lethal approaches for cancer treatment.