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Transfected Stable Cell Lines

Human NEK9 Knockout A-431 Cell Line

  • For research use only

Cat No.

ABC-KH041Y
Product Type

Knockout Stable Cell Line
Cell Type

Epithelial
Species

Human
Host Cell

A-431
Source Organ

Skin
Disease

Epidermoid Cancer
Storage

Liquid Nitrogen

Human NEK9 Knockout A-431 Cell Line by AcceGen enables study of mitotic regulation, chromosome instability and cancer cell proliferation mechanisms.

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  • Human NEK9 Knockout A-431 Cell Line
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Home | Transfected Stable Cell Lines | Human NEK9 Knockout A-431 Cell Line

Description

Human NEK9 Knockout A-431 Cell Line is generated from human epidermoid carcinoma A-431 cells through CRISPR/Cas9-mediated disruption of the NIMA-Related Kinase 9 (NEK9) gene, a serine/threonine kinase regulating mitotic spindle formation, chromosome segregation, and cell cycle progression. This knockout model exhibits severe mitotic defects including multipolar spindles and chromosome misalignment while maintaining A-431 cells’ characteristic epithelial morphology. The cell line provides a powerful tool for studying centrosome amplification, genomic instability, and cancer aneuploidy, with particular relevance to squamous cell carcinoma research involving PLK1-dependent mitotic regulation. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and functional phospho-proteomic analysis demonstrating loss of NEK9-mediated RCC2 phosphorylation. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.

Species

Human
Cat.No

ABC-KH041Y
Product Category

Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Epithelial
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Skin
Disease

Epidermoid Cancer
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Knockout Stable Cell Line
Host Cell

A-431
Gene Info

NEK9
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • The Human NEK9 Knockout A-431 Cell Line is a valuable model for studying mitotic regulation and chromosomal stability in epithelial cancers. This engineered system enables investigation of the NIMA-related kinase’s role in spindle assembly and cell cycle progression. Researchers utilize this knockout line to examine disrupted microtubule dynamics and aberrant mitotic checkpoint signaling in squamous cell carcinoma. The cell line provides a controlled platform for evaluating NEK9-targeted therapies and identifying synthetic lethal interactions in cancer treatment.

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