| Species |
Human
|
| Cat.No |
ABC-KH041Y
|
| Quality Control |
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
|
| Product Category |
Transfected Stable Cell Lines |
| Size/Quantity |
1 vial
|
| Cell Type |
Epithelial
|
| Shipping Info |
Dry Ice
|
| Growth Conditions |
37 ℃, 5% CO2
|
| Source Organ |
Skin
|
| Disease |
Epidermoid Cancer
|
| Biosafety Level |
1
|
| Storage |
Liquid Nitrogen
|
| Product Type |
Knockout Stable Cell Line
|
| Host Cell |
A-431
|
| Gene Info |
NEK9
|
Description
Human NEK9 Knockout A-431 Cell Line is generated from human epidermoid carcinoma A-431 cells through CRISPR/Cas9-mediated disruption of the NIMA-Related Kinase 9 (NEK9) gene, a serine/threonine kinase regulating mitotic spindle formation, chromosome segregation, and cell cycle progression. This knockout model exhibits severe mitotic defects including multipolar spindles and chromosome misalignment while maintaining A-431 cells’ characteristic epithelial morphology. The cell line provides a powerful tool for studying centrosome amplification, genomic instability, and cancer aneuploidy, with particular relevance to squamous cell carcinoma research involving PLK1-dependent mitotic regulation. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and functional phospho-proteomic analysis demonstrating loss of NEK9-mediated RCC2 phosphorylation. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.
