Human NEK9 Knockout A-431 Cell Line

Description

Human NEK9 Knockout A-431 Cell Line is generated from human epidermoid carcinoma A-431 cells through CRISPR/Cas9-mediated disruption of the NIMA-Related Kinase 9 (NEK9) gene, a serine/threonine kinase regulating mitotic spindle formation, chromosome segregation, and cell cycle progression. This knockout model exhibits severe mitotic defects including multipolar spindles and chromosome misalignment while maintaining A-431 cells’ characteristic epithelial morphology. The cell line provides a powerful tool for studying centrosome amplification, genomic instability, and cancer aneuploidy, with particular relevance to squamous cell carcinoma research involving PLK1-dependent mitotic regulation. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and functional phospho-proteomic analysis demonstrating loss of NEK9-mediated RCC2 phosphorylation. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.

Application

• FOR RESEARCH USE ONLY
  

• The Human NEK9 Knockout A-431 Cell Line is a valuable model for studying mitotic regulation and chromosomal stability in epithelial cancers. This engineered system enables investigation of the NIMA-related kinase’s role in spindle assembly and cell cycle progression. Researchers utilize this knockout line to examine disrupted microtubule dynamics and aberrant mitotic checkpoint signaling in squamous cell carcinoma. The cell line provides a controlled platform for evaluating NEK9-targeted therapies and identifying synthetic lethal interactions in cancer treatment.