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Human PIK3CA (P539R) BAF3 Cell Line

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Home | Transfected Stable Cell Lines | Overexpression Stable Cell Lines | Human PIK3CA (P539R) BAF3 Cell Line
Species

Human
Cat.No

ABC-X0284C
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Lymphocytes
Shipping Info

Dry Ice
Growth Conditions

37 °C, 5% CO2
Source Organ

Lymphatic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Overexpression Stable Cell Lines
Host Cell

BAF3

Description

Human PIK3CA (P539R) BAF3 Cell Line is a genetically engineered model derived from murine pro-B Ba/F3 parental cells based on customers’ requirement. PIK3CA (P539R) BAF3 overexpression cell line is generated by stable integration of exogenous human PIK3CA gene with the P539R activating mutation. Lentiviral transduction is used for delivery, and cultures are maintained below passage 20. Validation is conducted by qRT-PCR.
Target
PIK3CA encodes the p110α catalytic subunit of PI3K, a central node in growth and survival pathways. The P539R mutation lies in the helical domain and enhances lipid kinase activity, contributing to oncogenesis in breast, colorectal, and endometrial cancers. AcceGen offers generation of stable overexpression cell lines targeting any gene of your interest. Polyclonal or monoclonal is optional based on customers’ research needs.

AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • For research use only

  • This model enables mechanistic investigation of PI3K activation, resistance to PI3K inhibitors, and serves as a platform for screening targeted therapies in PIK3CA-driven cancers.

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