Immortalized Human Microglia

Description

Immortalized Human Microglia are derived from purified human microglial cells isolated from normal adult brain tissue and immortalized with SV40 large T antigen lentiviral transduction. This allows for continuous proliferation and extended lifespan. For optimal performance, these cells are cryopreserved at early passages. Under resting conditions, they exhibit typical ramified microglial morphology and retain key markers like Iba1 and CD68, confirmed by flow cytometry and immunocytochemistry. Functionally, these cells participate in brain surveillance, immune responses, synaptic pruning, and tissue repair. Upon exposure to LPS or β-amyloid, they become activated, secreting cytokines (e.g., IL-1β, TNF-α) and nitric oxide, and undergo morphological changes. They are ideal for studying human microglial cell line behavior, microglial cells activity, and mechanisms related to microglia function in neuroinflammation, neurodegenerative diseases, and drug discovery.

Application

• Microglia research has long been constrained by the availability of primary human sources. Immortalized Human Microglia (SV40 cell line) offers a consistent and accessible model that allows the establishment of in vitro models for studying the physiological and pathological properties of microglia cells. They can also be used to study neuron-microglia interactions and mechanisms underlying neurodegenerative and neuroinflammatory conditions