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Immortalized Cell Lines

Immortalized Mouse Microglia

  • For research use only

Cat No.

ABI-TC3325
Product Type

Immortalized Cell Line
Cell Type

Microglia
Species

Mouse
Growth Conditions

37 ℃, 5% CO2
Source Organ

Brain
Disease

Normal
Storage

Liquid Nitrogen

Immortalized mouse microglia from C57BL/6J retain Iba1, P2RY12, TGFβR; non-tumorigenic, great for neuroinflammation, HIV latency, and microglial function.

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Home | Immortalized Cell Lines | Immortalized Mouse Microglia

Description

The Immortalized Mouse Microglia cell line is derived from neonatal C57BL/6 mouse brain and transformed using SV40 large T antigen to extend proliferative capacity while preserving microglial phenotype. Maintaining typical microglial features, these cells show exhibit small, ramified or amoeboid morphology depending on activation state, and grow as adherent monolayers in culture. They express canonical microglial markers including Iba1, CD11b, CX3CR1, and TMEM119. Functionally, they retain phagocytic activity, secrete pro- and anti-inflammatory cytokines (e.g., TNF-α, IL-6, IL-10), and respond to stimuli such as LPS, ATP, and IFN-γ. This line is widely used to model neuroinflammation, neurodegeneration, and host-pathogen interactions in the CNS. Their genetic and functional stability across passages makes them ideal for reproducible in vitro studies of microglial behavior in aging, Alzheimer’s disease, multiple sclerosis, and brain injury.

Species

Mouse
Cat.No

ABI-TC3325
Product Category

Immortalized Cell Lines
Size/Quantity

1 vial
Cell Type

Microglia
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Brain
Disease

Normal
Storage

Liquid Nitrogen
Product Type

Immortalized Cell Line
Immortalization Method

Spontaneous immortalization
Morphology

Heterogenous; spindle, multi-polar, spherical, flat
Quality Control

1) Functional assays such as the nitrate assay was performed to assess nitric oxide production; 2) ELISA was used to determine the TNFα secretion after inflammatory stimulation; 3) Western blot and immunocytochemistry were used to confirm the expression of microglial specific markers such as CD68 and Iba1 and M1/M2 phenotype-associated protein expression (COX-2/iNOS and Arg-1) after stimulation; 4) Aβ1-42 or E. coli-derived bioparticles uptake was used to determine phagocytic activity of SIM-A9 cells.

Application

  • Immortalized mouse microglia closely mimic primary microglia and are essential for studying microglial functions such as migration, proliferation, and phagocytosis. Their activation by HIV-Tat enables investigation of HIV latency and regulatory mechanisms. These cells provide a robust model for elucidating neuroinflammatory processes and advancing therapeutic research in HIV-associated and other neurodegenerative diseases.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

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