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| Product Code | L6-H2 |
| Species | Rat |
| Cat.No | ABC-TC0575 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Rat Fibroblas Lymphocytes Cell Lines |
L6-H2 is a non-myogenic cell line and a sub-clone of the rat myogenic L6 cell line. It was originally derived from the skeletal muscle of a newborn rat. L6-H2 cells display a mix of fibroblast-like and rounded morphologies and exhibit loosely adherent culture properties. They are perpetuated in a continuous culture as a monolayer. Unlike their myogenic counterparts, L6-H2 cells do not form myotubes and are capable of proliferation in soft agar. For subculturing, cells are typically split at ratios ranging from 1:10 to 1:20 using trypsin/EDTA and seeded at a density of 2-4×10^4 cells per square centimeter.
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L6-H2 cells serve as a valuable tool in the myogenic research, primarily functioning as a negative control in studies involving the L6 cell line and its various subclones. They are instrumental in elucidating the characteristics of myogenic differentiation by providing a baseline for receptor expression, chemical stimulation, and signal transduction studies. By contrasting the behavior of L6-H2 cells with myogenic variants, researchers can gain critical insights into the mechanisms govern muscle cell differentiation, deepening our understanding of this intricate process.
