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| Product Code | LL/2 (LLc1); LL/2; LL2; LLC1; LLC; Lewis lung carcinoma line 1; Lewis lung carcinoma; Lewis Lung Cancer; Lewis-Lung; Lewis Lung |
| Species | Mouse |
| Cat.No | ABC-TC0591 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Lung |
| Disease | Lung Cancer |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Lung Cancer Cell Lines |
The Lewis lung carcinoma line 1 (LLC1), also known as LL/2, is a murine lung cancer cell line originally derived from a primary tumor in a C57BL/6 mouse. This syngeneic, immunocompetent model remains highly tumorigenic and metastatic, faithfully replicating the behavior of primary Lewis lung carcinoma, including lung colonization. LLC1 cells exhibit epithelial-like adherent morphology, grow efficiently in RPMI-1640 with 2% FCS or DMEM with 10% FBS. They are chromosomally abnormal and express both cytokeratin and vimentin, indicating epithelial-mesenchymal characteristics. These cells are tested negative for mycoplasma, bacteria, fungi, HIV, HBV, and HCV. LLC1 is widely used in metastasis, chemotherapy, and immunotherapy research, providing a reliable and reproducible murine model for preclinical lung cancer studies under physiologically relevant conditions.
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LL-2(LLC1) cells have broad applications in cancer research. They are useful in focus assays to assess drug-triggered cell death, providing insights into the impacts of different agents on cancer cell viability. Additionally, LL-2(LLC1) cells are commonly employed in syngeneic animal models to assess the efficacy of anticancer treatments in vivo. These models enable researchers to study treatment effectiveness and potential side effects in a controlled environment. Furthermore, LL-2(LLC1) cells are valuable in identifying key factors in human cancer. Researchers can test effects of antibodies or blockers on LL-2(LLC1) cells, shedding light on potential therapeutic targets and aiding in the development of targeted cancer therapies. Overall, LL-2(LLC1) cells serve as valuable tools for studying drug action, therapy results, and molecular targets in cancer biology.
The LL/2(LLc1) cells exhibit heterogeneous morphology, with both epithelial-like cells and round cells present simultaneously in varying proportions. Cells are loosely attached to the culture vessel. There may be a small number of floating cells; when adherent cells are abundant, the suspension cells can be discarded.
Be careful to control the cell density to avoid it being too high, as long-term high-density culture can lead to cell stacking or clustering.
The LL/2 cells are highly tumorigenic and can form tumors and metastases when implanted into immunocompromised or syngenic C57BL/6 mice. However, they are weakly metastatic in mice and have been described as non-responsive to checkpoint blockade immunotherapy.
