For research use only
| Cat No. | ABC-TC0639 |
| Product Type | Human Skin Cell Lines |
| Cell Type | Epithelial |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Breast |
| Product Code | MCF12A |
MCF-12A is non-tumorigenic mammary epithelial line, diploid karyotype, low ER/HER2, expresses CK18/19 and E-cadherin, model for benign breast studies.
MCF-12A is a non-tumorigenic human mammary epithelial cell line originally isolated from the fibrocystic breast tissue of a 60-year-old Caucasian female. MCF-12A exhibits epithelial-like morphology and adherent growth characteristics in monolayer cultures. Crytogenetic analysis confirms a normal diploid karyotype (46, XX) and the cell line is devoid of common oncogenic mutations typically associated with malignancy. This cell line expresses epithelial markers, including Cytokeratin 8, 14, and 18 (CK8, CK14, CK18), but tests negative for CK19, as confirmed by immunohistochemistry. This profile makes MCF-12A a suitable candidate for studying non-malignant breast cancer related disease.
| Product Code | MCF12A |
| Species | Human |
| Cat.No | ABC-TC0639 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Breast |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Skin Cell Lines |
MCF-12A cell line serve as a versatile platform for investigating the biology of normal human mammary epithelium and for comparative studies in breast cancer research, They are widely used to explore gene function, signal transduction pathways, and transcriptional regulation in both 2D and 3D culture systems. MCF-12A is valuable for 3D culture studies, in 3D environments, MCF-12A cells facilitates detailed investigations into cell proliferation, morphogenesis and differential gene expression patterns between non-malignant and malignant cells. Their stable, non-cancerous phenotype provides a crucial baseline for assessing the molecular and phenotypic changes that accompany breast tumor progression, drug responses, and microenvironmental influences.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
