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| Product Code | MCF12A |
| Species | Human |
| Cat.No | ABC-TC0639 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Breast |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Skin Cell Lines |
MCF-12A is a non-tumorigenic human mammary epithelial cell line originally isolated from the fibrocystic breast tissue of a 72-year-old Caucasian female. MCF-12A exhibits epithelial-like morphology and adherent growth characteristics in monolayer cultures. Crytogenetic analysis confirms a normal diploid karyotype (46, XX) and the cell line is devoid of common oncogenic mutations typically associated with malignancy. This cell line express epithelial markers such as Cytokeratin 18/19 (CK18/CK19), and E-cadherin, while exhibiting low levels of estrogen receptor (ER) and HER2 as assessed by immunohistochemistry. This profile makes MCF-12A a suitable candidate for studying non-malignant breast cancer related disease.
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MCF-12A cell line serve as a versatile platform for investigating the biology of normal human mammary epithelium and for comparative studies in breast cancer research, They are widely used to explore gene function, signal transduction pathways, and transcriptional regulation in both 2D and 3D culture systems. MCF-12A is valuable for 3D culture studies, in 3D environments, MCF-12A cells facilitates detailed investigations into cell proliferation, morphogenesis and differential gene expression patterns between non-malignant and malignant cells. Their stable, non-cancerous phenotype provides a crucial baseline for assessing the molecular and phenotypic changes that accompany breast tumor progression, drug responses, and microenvironmental influences.
