Mouse Lymphatic Endothelial Cells (MLECs) are primary cells isolated from healthy murine lymphatic tissues. These cells exhibit a cobblestone-like or oak-leaf morphology and display adherent growth properties. These cells retain key physiological functions including maintaining fluid and protein homeostasis, regulating immune responses, and facilitating lymphocyte recirculation. They are involved in various pathological processes like lymphedema, lymphangitis, and tumor metastasis, largely due to their role in lymphatic remodeling and immune modulation. MLECs express lymphatic-specific markers, including CD31 and LYVE-1. As primary cells, MLECs have limited proliferative capacity (typically ≤5 passages) and require optimized culture conditions to preserve functionality.
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