Rat Oligodendrocyte Precursor Cells (OPCs) are derived from the optic nerve of 7-day-old healthy rats and used at low passages (typically P1–P3) to maintain lineage fidelity. These non-tumorigenic, non-metastatic neural precursors exhibit bipolar or multipolar morphology and grow adherently in vitro. They can differentiate into oligodendrocytes or astrocytes depending on culture conditions. OPCs express A2B5, O4, and NG2, detected via immunofluorescence or flow cytometry, and lack MBP/PLP expression. They proliferate in response to PDGF and bFGF. Karyotype analysis is not reported, and viral testing (e.g., Mycoplasma, LDV) is not specified. No maximum passage limit is detailed, but proliferative potential declines with extended culture. These cells are a standard model for demyelinating diseases like multiple sclerosis, ideal for remyelination and CNS repair research.
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