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Species | Rat |
Cat.No | ABC-TC4233 |
Quality Control | All cells test negative for mycoplasma, bacteria, yeast, and fungi. |
Product Category | Primary Cells |
Size/Quantity | 1 vial |
Cell Type | Epithelial |
Shipping Info | Dry Ice |
Growth Conditions | 37 ℃, 5% CO2 |
Source Organ | Tracheal |
Disease | Normal |
Biosafety Level | 1 |
Storage | Liquid Nitrogen |
Product Type | Rat Primary Cells |
Rat tracheal epithelial cells are derived from the tracheal tissue of Sprague-Dawley rats in the laboratory. These cells can develop into mucociliary, mucous, or squamous phenotypes, with the mucociliary phenotype being the most faithful to the in vivo tracheal epithelial morphology. Following primary culture, these cells are cryopreserved. The squamous phenotype is characterized by the loss of type II transglutaminase and keratin 18, accompanied by the expression of squamous markers such as type I transglutaminase and keratin 13. Repeated freezing and thawing should be avoided during the culture process.
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Rat tracheal epithelial cells offer a useful in vitro system for studying tracheal cell biology. They can be cultured into different phenotypes, allowing detailed analysis of cell proliferation, differentiation, and function. RTE monolayers are especially valuable in exploring cellular transformation, including changes in growth factors and receptor expression, making them a key model in respiratory and airway biology.