Rat Tracheal Epithelial Cells

Description

Rat Tracheal Epithelial Cells are primary cells isolated from the epithelial lining of Sprague-Dawley rat trachea and cryopreserved after initial culture to preserve native properties. These cells exhibit pseudostratified columnar morphology, consisting of ciliated, goblet, and basal progenitor cells. They can differentiate into mucociliary, mucous, or squamous phenotypes, with the mucociliary phenotype closely resembling in vivo tracheal epithelium. Key marker expression includes cytokeratin-18, MUC5AC (mucin), and β-tubulin IV, highlighting their roles in mucociliary clearance and airway defense. Functionally, these cells maintain barrier integrity and actively participate in immune responses against inhaled pathogens and toxins. Their physiological relevance makes them a robust in vitro model for studying respiratory diseases such as asthma, COPD, and airway remodeling. Repeated freeze-thaw cycles should be avoided to preserve viability.