TR4 Knockout 293T Cell Line

Description

TR4 Knockout 293T Cell Line is generated from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the Testicular Receptor 4 (TR4/NR2C2) gene, an orphan nuclear receptor that regulates metabolic homeostasis, neural development, and reproductive functions. This knockout model exhibits altered transcriptional regulation of target genes while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line serves as a valuable tool for studying metabolic syndromes (including diabetes and fatty liver disease), neurodegenerative processes, and steroid hormone signaling, particularly in research involving PPARγ and LXR pathway crosstalk. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and functional luciferase reporter assays. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.