YTHDF2 Knockout 293T Cell Line

Description

YTHDF2 Knockout 293T Cell Line is generated from human embryonic kidney 293T cells using CRISPR/Cas9-mediated disruption of the YTH N6-Methyladenosine RNA Binding Protein 2 (YTHDF2) gene, a key reader protein that recognizes N6-methyladenosine (m6A) modifications and mediates mRNA stability and degradation. This knockout model exhibits altered RNA metabolism and prolonged mRNA half-life of m6A-modified transcripts while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line serves as an essential tool for studying epitranscriptomic regulation, stem cell differentiation, and cancer progression, particularly in research involving m6A-dependent RNA turnover and translational control. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and m6A-RNA immunoprecipitation assays. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.