Rat MicroRNA Agomir/Antagomir

About Rat MicroRNA Agomir/Antagomir

Rat miRNA Modulation for In Vivo Research

Rat MicroRNAs (rno-miR-) are a class of non-coding single-stranded RNA molecules about 22 nucleotides in length encoded by endogenous genes, which are involved in the regulation of post-transcriptional gene expression in the rat. Rat MicroRNA Agomir is a special-labeled and chemically modified double-stranded small RNA that mimics the rat endogenous miRNA to regulate the biological function of the target gene. Rat MicroRNA Antagomir is an especially chemically modified miRNA antagonist. Rat miRNA Agomir/Antagomir has higher stability/inhibitory effects in vivo and in vitro, and can overcome obstacles such as cell membranes and tissues in the body to enrich for target cells. In animal experiments, it can be administered by systemic or local injection, inhalation, feeding, etc. The effect can last for several weeks.

NOMENCLATURE

1. The miRNA mature body is abbreviated as miR, and then according to its species name, such as the human being abbreviated as hsa, and the order in which it was found is represented by Arabic numerals, such as hsa-miR-122.
2. Highly homologous miRNAs are followed by lowercase letters (a, b, c, ...), such as hsa-miR-34a, hsa-miR-34b, hsa-miR-34c, and so on.
3. MiRNAs with the same mature sequence are transcribed and processed from DNA sequences on different chromosomes, followed by Arabic numerals to indicate the difference, such as hsa-miR-199a-1 and hsa-miR-199a-2.
4. Generally, a miRNA precursor is about 70-80nt in length. It is likely that miRNAs are generated by the two arms, and they are named by "-5p" and "-3p". For example, hsa-miR-26b-5p and hsa-miR-26b-3p indicate that they are processed from the 5 'end arm and 3' end arm of the hsa-mir-26b precursor, respectively.

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