Technical Support
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Hot Products
- In-Stock Tumor Cell Lines
- Human Orbital Fibroblasts
- Human Microglia
- Human Pulmonary Alveolar Epithelial Cells
- Human Colonic Fibroblasts
- Human Type II Alveolar Epithelial Cells
- Human Valvular Interstitial Cells
- Human Thyroid Epithelial Cells
- C57BL/6 Mouse Dermal Fibroblasts
- Human Alveolar Macrophages
- Human Dermal Fibroblasts, Adult
- Human Lung Fibroblasts, Adult
- Human Retinal Muller Cells
- Human Articular Chondrocytes
- Human Retinal Pigment Epithelial Cells
- Human Pancreatic Islets of Langerhans Cells
- Human Kidney Podocyte Cells
- Human Renal Proximal Tubule Cells
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What is the passage limit for Aged Mouse Bone Marrow-Derived Endothelial Cells?
These cells can be passaged 3-5 times.
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What is the maximum passage number for Bovine Muscle Satellite Cells?
Under recommended conditions, these cells can be passaged 3-5 times at a split ratio of 1:2 . However, proliferation capacity varies between different donors.
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What method was used to immortalize Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells?
These cells are typically immortalized using SV40. Alternatively, hTERT can be used upon request. However, all immortalized stem cells may lose some differentiation potential.
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How is quality control performed for immortalized cell lines?
After immortalization, AcceGen verifies vWF expression and checks cell morphology.
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What is the recommended bacterial infection protocol for Human Vaginal Epithelial Cells?
When preparing the bacterial inoculum, the bacterial concentration should be adjusted to achieve an appropriate multiplicity of infection (MOI). Before the experiment, Human Vaginal Epithelial Cells should be cultured to approximately 85% confluence. Additionally, attention should be paid to the culture medium, particularly whether antibiotics are included and whether these antibiotics could affect bacterial growth.
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How are Human Kidney Podocyte Cells tested?
These cells are tested using immunofluorescence staining and qPCR.
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How are Human Kidney Podocyte Cells isolated?
AcceGen's podocytes are isolated using the FACS method. Kidney tissue is first processed to remove unwanted components, followed by filtration to collect the filtrate. The filtrate is then passed through filters of different pore sizes to collect the Kidney Podocyte Cells, which are subsequently resuspendedin the specialized culture medium, plated, and identified using immunofluorescence staining.
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What is the biosafety level of the cells?
The biosafety level of these cells is BSL-2.
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What dissociation reagent should I use to detach the cells?
For Reprogrammed Human iPS Cell , Accutase is the recommended dissociation reagent.
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What type of FBS should I purchase for this product?
We recommend purchasing ready-to-use FBS, which should be filtered and sterilized using a 0.22 µm membrane before use.
