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Product Code | Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo |
Species | Human |
Cat.No | ABC-TC441S |
Product Category | Tumor Cell Lines |
Size/Quantity | 1 vial |
Cell Type | Epithelial |
Shipping Info | Dry Ice |
Growth Conditions | 37 ℃, 5% CO2 |
Source Organ | Kidney |
Storage | Liquid Nitrogen |
Product Type | Human Kidney Cell Lines |
293T is a derivative cell line of the human embryonic kidney cell line HEK293, originally obtained by transforming HEK293 cells with adenovirus 5 DNA, and can be generated by the SV40 T antigen transfection. The 293T cells exhibit epithelial-like morphology and adherent culture characteristics. The stemline karyotype of this cell line is hyperdiploid, with a modal chromosome number of 64. 293T is an aneuploid cell line, displaying chromosomal counts within the near-triploid range. This cell line is tumorigenic and has a high transfection efficiency and is commonly used for lentivirus packaging, gene expression, and signal transduction studies.
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293T cells serve as a versatile platform for viral vector production (including retroviruses, lentiviruses, and adenoviruses) by efficiently packaging SV40 origin-containing vectors. Their high transferability supports transient gene expression studies and transfection assay optimization while enabling large-scale recombinant protein production for research/therapeutics. These cells additionally facilitate pseudovirus generation for viral entry/replication studies and drug discovery research targeting ion channels, protein interactions, and post-translational modifications, along with applications in RNA interference, cell cycle progression, and cancer metastasis.