For research use only
| Cat No. | ABC-TC441S |
| Product Type | Human Kidney Cell Lines |
| Cell Type | Epithelial |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Kidney |
| Product Code | Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo |
293T cells are HEK293 derivatives, epithelial, tumorigenic, highly transfectable, used in lentivirus packaging, gene expression, and signaling studies.
293T is a human embryonic kidney (HEK)-derived cell line generated by stably transfecting HEK293 cells with the SV40 large T antigen, enabling episomal replication of plasmids containing the SV40 origin. Derived from a female fetus, 293T cells exhibit adherent, epithelial morphology and a hyperdiploid to near-triploid karyotype, typically containing 64–70 chromosomes. The expression of SV40 large T antigen supports high-efficiency transfection, making 293T a preferred host for transient gene expression, lentiviral vector production, and protein overexpression. The line is resistant to G418 and neomycin, allowing for selection in stable transfection protocols. Additionally, 293T cells demonstrate robust growth and are widely used in signal transduction studies, AP fusion protein expression, and gene function assays, making them a versatile and indispensable tool in molecular biology and biotechnology research.
| Product Code | Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo |
| Species | Human |
| Cat.No | ABC-TC441S |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Kidney |
| Biosafety Level | 2 |
| Storage | Liquid Nitrogen |
| Product Type | Human Kidney Cell Lines |
293T cells serve as a versatile platform for viral vector production (including retroviruses, lentiviruses, and adenoviruses) by efficiently packaging SV40 origin-containing vectors. Their high transferability supports transient gene expression studies and transfection assay optimization while enabling large-scale recombinant protein production for research/therapeutics. These cells additionally facilitate pseudovirus generation for viral entry/replication studies and drug discovery research targeting ion channels, protein interactions, and post-translational modifications, along with applications in RNA interference, cell cycle progression, and cancer metastasis.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
