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Tumor Cell Lines

293T

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Tissue: kidney, embryonic; Transformed by: SV40 large T antigen; Original line: 293. Used for transfection assays and as packaging cells. The cell line can be transiently transfected and give extremely high levels of expression of AP fusion proteins. G418 resistant, neomicine resistant. The cell line is known also as HEK293T.
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Product Code

Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo

Species

Human

Cat.No

ABC-TC441S

Product Category Tumor Cell Lines
Size/Quantity

1 vial

Cell Type

Epithelial

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Kidney

Storage

Liquid Nitrogen

Product Type

Human Kidney Cell Lines

Description

293T is a derivative cell line of the human embryonic kidney cell line HEK293, originally obtained by transforming HEK293 cells with adenovirus 5 DNA, and can be generated by the SV40 T antigen transfection. The 293T cells exhibit epithelial-like morphology and adherent culture characteristics. The stemline karyotype of this cell line is hyperdiploid, with a modal chromosome number of 64. 293T is an aneuploid cell line, displaying chromosomal counts within the near-triploid range. This cell line is tumorigenic and has a high transfection efficiency and is commonly used for lentivirus packaging, gene expression, and signal transduction studies.

Citation

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Application

  • 293T cells serve as a versatile platform for viral vector production (including retroviruses, lentiviruses, and adenoviruses) by efficiently packaging SV40 origin-containing vectors. Their high transferability supports transient gene expression studies and transfection assay optimization while enabling large-scale recombinant protein production for research/therapeutics. These cells additionally facilitate pseudovirus generation for viral entry/replication studies and drug discovery research targeting ion channels, protein interactions, and post-translational modifications, along with applications in RNA interference, cell cycle progression, and cancer metastasis.

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