For research use only
| Cat No. | ABC-TC0028 |
| Product Type | Human Melanoma Cell Lines |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Disease | Amelanotic Melanoma |
| Product Code | A-375SM; A375-SM |
Tumorigenecity: Isoenzyme: Histopathology: melanoma, highly metastaticSubculture: Split ratio: Media change: Reverse transcritase: Production
A375SM cell line is a highly metastatic subline of the human malignant melanoma cell line A375, originally derived from a skin lesion of a 54-year-old female patient. It was selected through in vivo passaging in nude mice. A375SM exhibits epithelial-like, polygonal morphology, grows as adherent monolayers, and displays a hypotriploid karyotype. The cells harbor the BRAF V600E mutation and show elevated motility and invasiveness compared to A375 and A375P, forming more and larger colonies in soft agar. Proteomic analyses reveal upregulated mesenchymal markers such as vimentin and nestin. In xenograft models, A375SM robustly forms lung metastases, making it a relevant model for metastatic melanoma. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.
| Product Code | A-375SM; A375-SM |
| Species | Human |
| Cat.No | ABC-TC0028 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Disease | Amelanotic Melanoma |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Melanoma Cell Lines |
A375SM serves as a robust model for melanoma metastasis and therapeutic screening. It is widely used in studies dissecting EMT regulation, YB-1 signaling, and cytoskeletal dynamics driving invasion. TRPV4-dependent actin reorganization was shown to promote A375SM metastasis, which could be reversed by baicalin treatment. Additionally, A375SM xenograft models are employed to evaluate anti-metastatic therapies, demonstrating significant suppression of lung colonization. This line is ideal for preclinical testing of drugs targeting metastatic pathways and microenvironment interactions.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
