Alpha TC1-9

Description

Alpha TC1‑9 is a murine pancreatic alpha-cell line cloned from a transgenic islet adenoma derived in (C57BL/6 × DBA/2 hybrid) mice expressing SV40 large T antigen under control of the rat preproglucagon promoter. These cells exhibit epithelial-like morphology, adherent growth properties and grow as single cells or loosely attached clusters. Cytogenetic analysis reveals aneuploidy, yet this line retains key functional alpha-cell characteristics, including glucagon secretion regulated by extracellular glucose concentrations. This line is heterozygous for MHC alleles (H‑2ᵇ/h‑2ᵈ), with induced class II MHC expression upon IFN-γ treatment. The cells show modest resistance to combined cytokine (IL‑1 + IFN‑γ) cytotoxicity relative to beta-cell lines. Unlike its progenitor, Alpha TC1, this subclone does not express insulin or preproinsulin mRNA, making it a specific model for alpha-cell function without beta-cell interference. The cell line serves as a critical tool for studying alpha-cell physiology, chemical toxicity, and transdifferentiation mechanisms.

Application

• The Alpha TC1-9 cell line offers a versatile platform for exploring multiple aspects of pancreatic alpha-cell biology. It facilitates detailed investigations of glucagon biosynthesis, biosynthesis, glucose sensing and cytokine responsiveness, contributing to a comprehensive understanding of the intricate regulation of glucose sensing and glucagon secretion. Furthermore, it plays a crucial role in elucidating the impacts of metabolism-disrupting chemicals on pancreatic endocrine function. Importantly, this cell line supports research into the transdifferentiation potential of alpha-cells into beta-cells, thus offering promising avenues for beta-cell regeneration and in diabetes research and therapeutic development.