ATP7B Knockout 293T Cell Line

Description

ATP7B Knockout 293T Cell Line is generated from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the ATPase Copper Transporting Beta (ATP7B) gene, a critical copper-transporting P-type ATPase that regulates cellular copper homeostasis and biliary copper excretion. This knockout model exhibits defective copper transport and accumulation while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line serves as an essential tool for studying Wilson’s disease pathogenesis, copper metabolism disorders, and heavy metal toxicity mechanisms, particularly in research involving ceruloplasmin processing and liver-specific copper transport. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and functional copper accumulation assays. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.