Bak Knockout 293T Cell Line

Description

Bak Knockout 293T Cell Line is generated from human embryonic kidney 293T cells using CRISPR/Cas9 technology to disrupt the BCL2-antagonist/killer 1 (Bak) gene, a key pro-apoptotic protein in the BCL-2 family that regulates mitochondrial outer membrane permeabilization. This knockout model exhibits resistance to intrinsic apoptosis stimuli while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line provides an essential tool for studying apoptotic pathways, cancer cell survival mechanisms, and drug resistance, particularly in research involving mitochondrial-mediated cell death. Maintained at low passage numbers (<P20) with stable genotype, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and functional apoptosis assays. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.