Canine Skeletal Muscle Cells

Description

Canine Skeletal Muscle Cells (CnSkMC) are primary myoblasts isolated from clinically healthy, pathogen-free dog skeletal muscle tissue via enzymatic dissociation. CnSkMC display the typical elongated spindle morphology of myogenic progenitors and express markers such as desmin and MyoD, confirmed through immunocytochemistry. Under appropriate differentiation conditions, these cells are capable of forming multinucleated myotubes and expressing mature muscle markers such as myosin heavy chain (MyHC). Karyotype analysis confirms chromosomal stability, and donor animals are screened to be free of neuromuscular disorders. To ensure the quality of these primary cells, repeated freeze-thawcycles are not recommended due to the fragile nature of these cells. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.