Caspase-8 Colorimetric Assay Kit

Description

Caspases are a family of proteases involved in apoptosis, encompassing over ten members. Caspase-8, also known as FLICE, MACH, or Mch5, typically exists as a zymogen and is activated during apoptosis. It is considered an upstream caspase in the apoptotic pathway. During Fas receptor- and TNFR-1-mediated apoptosis, caspase-8 is activated to form dimers, which in turn activate downstream caspases 4, 6, 9, and 10. The assay is based on the specific hydrolysis of the peptide substrate Ile-Glu-Thr-Asp-p-nitroanilide (IETD-pNA) by caspase-8. The released free nitroanilide (pNA) has a maximum absorbance at 405 nm.