CIITA Knockout THP-1 Cell Line

Description

CIITA Knockout THP-1 Cell Line is derived from the human monocytic THP-1 cell line and generated via CRISPR/Cas9-mediated gene editing to disrupt the Class II Major Histocompatibility Complex Transactivator (CIITA) gene. THP-1 cells exhibit suspension growth with round morphology and are widely used to study macrophage differentiation, immune regulation, and inflammatory responses. This knockout model demonstrates loss of CIITA, a master regulator of MHC class II expression, making it a valuable tool for research in antigen presentation, T-cell activation, and autoimmune diseases. The edited cells are maintained at low passage numbers (<P20) to ensure genetic stability, and knockout efficiency is confirmed through PCR, Sanger sequencing, or Western blot. The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.