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EBC-1/original; EBC1






1 vial

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Dry Ice


Liquid Nitrogen


EBC-1 cells are a human lung squamous cell carcinoma line derived from explant cultures of various types of lung carcinomas, including squamous cell, adeno-, and small cell carcinoma. When transplanted into hamsters, EBC-1 cells exhibit the characteristic feature of metastasizing to the lungs. These cells possess a p53 mutation (p.Glu171Ter, c.511G>T) and display heterogeneity in EGFR gene mutations. Additionally, EBC-1 cells exhibit amplification of the Met gene and overexpression of Met receptors, which are involved in the growth, invasion, and metastasis of the tumor. EBC-1 cells exhibit an epithelial-like morphology and adherence in culture. Subculturing is performed using a 1:8 split ratio and 0.25% Trypsin for detachment.


Why choose EBC-1 from AcceGen?

EBC-1 cells from AcceGen possess several key features. They are sterile, ensuring the absence of bacteria, fungi, and mycoplasma contamination. These cells are prepared by professional operators following optimal standards, guaranteeing the reliability and reproducibility of experimental results. Rigorous quality control measures are implemented to maintain the authenticity and characteristics of EBC-1 cells. Moreover, these cells exhibit high viability, ensuring their suitability for various experimental assays and long-term culturing. These features make EBC-1 cells a reliable and valuable resource for studying lung squamous cell carcinoma and related research.

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Citation Guide

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EBC-1 cells, when implanted into hamsters, serve as a valuable model for studying human lung cancer. Researchers can utilize this model to investigate the mechanisms underlying metastasis and evaluate the chemosensitivity of anticancer agents. The EBC-1 model enables the exploration of new targets for cancer therapy and allows the testing of relevant inhibitors to determine their ability to suppress pulmonary metastasis. This cell line provides a platform for studying the efficacy of potential therapeutic interventions and aids in the development of novel treatment strategies for lung cancer.

Growth Conditions

37 ℃, 5% CO2

QuestionsthreeWhat is the recommended seeding density?
Product Type

Human Lung Cancer Cell Lines

Product Image AcceGen Frozen Cells & Cell Lines 1 vial

Frequently Asked Questions

  • What is the origin of EBC-1 cell line?

    These cells are derived from a Japanese male with non-small cell lung carcinoma.

  • How often should the medium be changed?

    The medium should be changed every 2-3 days.

  • What is the recommended seeding density?

    Approximately 1-2×10^4 cells/cm².

  • Why are my EBC-1 cells not adhering to the culture vessel?

    Surface Quality: Ensure the culture vessels are tissue culture-treated.


    Coating: Use extracellular matrix proteins like collagen or fibronectin to enhance adhesion.


    Passage Number: Use cells with a lower passage number to avoid adhesion issues due to cellular senescence.

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