HighQC™ Human Colon Stem Cell

Description

HighQC™ Human Colon Stem Cells are adult stem cells isolated from healthy human colon tissue and cultured under defined, serum-free conditions to maintain their self-renewal and multipotency. These cells exhibit a crypt-like, epithelial morphology and express key stem cell markers including Oct 4, ESA, SSEA 3/4, telomerase, Ki67, Cdx2, HNF-1, confirmed by RT-qPCR and flow cytometry. They retain the ability to generate all major epithelial lineages of the colon, such as absorptive enterocytes, goblet cells, and enteroendocrine cells. These cells model the in vivo behavior of intestinal crypt stem cells and reflect normal epithelial turnover and regeneration. Under 3D culture, they form colonic organoids that recapitulate in vivo architecture and functionality. Their stable proliferation and differentiation potential make them ideal for modeling gut homeostasis, epithelial repair, and disease onset. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from HIV-1, HBV, HCV, syphilis, mycoplasma, fungi, yeast, and bacteria.