HighQC™ Human IPSC From Activated Fibroblast From Primary Keratocytes-Keratoconus

Description

HighQC™ Human IPSC From Activated Fibroblast From Primary Keratocytes-Keratoconus is a disease-specific human induced pluripotent stem cell line. It retains pluripotency for keratoconus corneal mechanism research. It was generated by reprogramming activated fibroblasts that were originally derived from primary keratocytes of a donor diagnosed with Keratoconus, a progressive corneal thinning disorder. The reprogramming was performed using non-integrating episomal plasmid method. The resulting iPSCs (induced pluripotent stem cells) exhibit adherent growth with well-defined borders and a high nucleus-to-cytoplasm ratio. These Keratoconus iPSCs retain the fundamental capacity for trilineage differentiation into cell types of the ectoderm, mesoderm, and endoderm germ layers. Core pluripotency marker SSEA-4 is expressed. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.