HighQC™ Human iPSC Line (Episomal, CB)

Description

The HighQC™ Human iPSC Line (Episomal, CB) is a human induced pluripotent stem cell line generated from cord blood mononuclear cells of a healthy newborn. Reprogramming of these cells was achieved using episomal plasmids expressing OCT4, SOX2, KLF4, L-MYC, and Lin28. The cells exhibit typical human embryonic stem cell morphology and they form compact colonies with a high nuclear-to-cytoplasmic ratio under standard ES cell culture conditions. These iPSCs retain a normal human karyotype, confirmed by G-banding, and are free from oncogenic mutations. They express key pluripotency markers including OCT4, SOX2, NANOG, SSEA-4, and TRA-1-60. These markers are essential for maintaining the pluripotent state of the cells and are commonly used to identify and characterize hiPSCs.

Application

• In the study of disease mechanisms, HighQC™ Human iPSC Line (Episomal, CB) can be used to simulate the pathological process of genetic or acquired diseases by directing differentiation into specific cell types (such as neurons, cardiomyocytes or hepatocytes). In terms of drug screening and toxicity testing, its differentiated functional cells can be used for high-throughput drug evaluation to improve the accuracy and efficiency of preclinical research.