HighQC™ Mouse Embryonic Stem Cell, From C57BL/6N Murine

Description

HighQC™ Mouse Embryonic Stem Cells are derived from healthy male C57BL/6N mouse blastocysts and display a typical undifferentiated round morphology with robust colony formation. They possess a normal male (XY) karyotype and express key pluripotency markers such as Oct-4 and Nanog, commonly detected via immunocytochemistry or qPCR. These ESCs are non-tumorigenic but exhibit full pluripotency, capable of contributing to germline chimera formation. Cells are cultured on either SNL7 fibroblast feeder layers or gelatin-coated plates with 10–15% serum to maintain their undifferentiated state. They are maintained under conditions suitable for long-term self-renewal. They are typically passaged up to 20 times while retaining pluripotency and genomic stability. They can differentiate into all three germ layers, supporting use in developmental biology. Viral screening (HIV, HTLV, mycoplasma) is recommended prior to use. These cells are ideal for gene editing, lineage tracing, and transgenic model creation.

Application

• Mouse Embryonic Stem Cells (ESCs), derived from C57BL/6N mice, have wide applications in stem cell and developmental biology. They serve as an excellent model for studying pluripotent stem cell expansion and differentiation when cultured in bioreactors. With their stable genome and high susceptibility to genetic modifications, mouse ESCs are valuable for generating large-scale mutant libraries, enabling phenotype-driven genetic screens. Moreover, the ease of genetic modification and their ability to be produced in large quantities provides a unique model for studying early mammalian development.