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Stem Cells

HighQC™ C57BL/6 Mouse Embryonic Stem Cells with GFP

  • For research use only

Cat No.

ABC-SC044G
Product Type

Animal Embryonic Stem Cells
Cell Type

Stem Cell
Species

Mouse
Growth Conditions

37 ℃, 5% CO2
Source Organ

Embryonic
Disease

Normal
Storage

Liquid Nitrogen

Explore HighQC™ C57BL/6 Mouse Embryonic Stem Cells with GFP for visual tracking in developmental studies, gene function analysis, and transgenic modeling.

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  • HighQC™ C57BL/6 Mouse Embryonic Stem Cells with GFP
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Home | Stem Cells | Embryonic Stem (ES) Cells | HighQC™ C57BL/6 Mouse Embryonic Stem Cells with GFP

Description

AcceGen’s HighQC™C57BL/6 Mouse ESCs/GFP are derived from the inner cell mass
of strain C57BL/6 Mouse blastocysts (at 3.5 dpc) and cultured on γ-ray irradiated
mouse embryonic fibroblasts as feeder cells in AcceGen’s Mouse ESC Growth Medium,
Cells have been transfected with a lentiviral construct with GFP.

Product Code

HighQC™ C57BL/6 Mouse Embryonic Stem Cells with GFP, C57BL/6 mESCs GFP, B6 Mouse ES Cells GFP, GFP-labeled C57BL/6 ESCs, Green Fluorescent B6 ESCs
Species

Mouse
Cat.No

ABC-SC044G
Product Category

Stem Cells
Size/Quantity

1 vial
Cell Type

Stem Cell
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Embryonic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Animal Embryonic Stem Cells
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Frequently Asked Questions

  • Which promoter drives GFP expression in HighQC™ C57BL/6 Mouse Embryonic Stem Cells with GFP, and is GFP expression stable during differentiation?

    GFP expression in HighQC™ C57BL/6 Mouse Embryonic Stem Cells with GFP is driven by the cytomegalovirus (CMV) promoter. The GFP expression cassette was introduced by random genomic integration via electroporation, followed by hygromycin selection to establish a stable cell line.

     

    GFP expression is stable during routine maintenance and expansion of the undifferentiated cells. However, GFP expression during differentiation may vary depending on the differentiated cell type and the activity of the CMV promoter, which can be influenced by lineage-specific regulatory mechanisms and transgene integration sites. Therefore, GFP expression should be evaluated in differentiated cells rather than assumed to remain unchanged.

     

    For best results, GFP expression should be monitored at different stages of culture and differentiation using flow cytometry (FACS) or fluorescence microscopy.

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