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HIT-T15

Product Name

HIT-T15

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Product Code

HIT T15; HIT.T15; HIT T-15; HITT15; HIT clone T15; Hamster Islet Transformed-Tioguanine resistant clone 15; HIT

Cat.No

ABC-TC0386

Species

Hamster

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

This line was established from a primary culture of Syrian hamster islet cells which were transformed with SV40.
The cells secrete up to 24ng of RI in per 10 exp6 cells per 24 hours(passage 60).
RI in secretion is stimulated by glucose and RI B, and is suppressed by somatostatin and glucocorticoids.
RI synthesis decreases with length of time in culture.Tumorigenecity: Isoenzyme: Histopathology: Subculture: Remove medium, add fresh 0.25 % Trypsin (enough to cover the monolayer) and let stand at room temperature until the cells detach.
Add fresh medium, aspirate and dispense into new flasks at about 1.3 X exp5 cells per sq.Cm.Split ratio: Media change: Reverse transcritase: ProductionRI.

Source Organ

Pancreas

Recommended Medium And Supplement

F12K + 15% FBS

Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

For research use only

Growth Conditions

37 ℃, 5% CO2

Cell Type

Epithelial

Growth Mode

Adherent

Product Type

Mouse Pancreas Cell Lines

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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