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| Product Code | huH-1; HUH-1; huH 1; HuH1; HUh1; HUH1; huH1 |
| Species | Human |
| Cat.No | ABC-TC0434 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial-like |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Liver |
| Disease | Hepatocellular Cancer |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Liver Cancer Cell Lines |
The Huh-1 cell line originates from the liver tissue of a patient with liver cancer. Morphologically, Huh-1 cells exhibit a polygonal or epithelial-like shape, with clear cell boundaries and a relatively high nuclear-to-cytoplasmic ratio. Epithelial cell characteristics such as tight junctions can be observed between the cells. Their growth mode is adherent. Under suitable culture conditions, the cells adhere tightly to the surface of the culture dish, growing in a monolayer or in local aggregates. Huh-1 cells retain some hepatocyte features, such as the expression of alpha-fetoprotein (AFP), but their albumin secretion and urea synthesis capacities may be limited compared to normal hepatocytes. They also display tumor-associated features, including the expression of potential epithelial-mesenchymal transition (EMT) markers (such as E-cadherin and N-cadherin) under certain conditions. Additionally, they display metabolic activity typical of cancer cells, such as enhanced glycolysis and lipid metabolism.
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Huh-1 cell line is widely used to explore key signaling pathways in liver cancer, such as abnormal activation of Wnt/β-catenin, PI3K/AKT and MAPK pathways, and the effects of tumor suppressor gene (such as TP53) mutations on tumor progression. In addition, this cell line can be used to study the metastasis mechanism of liver cancer, especially the regulatory role of epithelial-mesenchymal transition (EMT) and angiogenesis-related factors.
huH-1 cells were originally isolated from liver of hepatocellular carcinoma, HBs-antigen carrier patient. Modal chromosome number is 69.
The culture medium recommended for huH-1 cells is composed of High Glucose DMEM supplemented with appropriate additives to support optimal cell growth and maintenance, add 10% FBS.
Hepatitis B virus (HBV) is detected in the huH-1 cell line with a high transcriptional activity. The huH-1 is one of the cell lines that used extensively to study HBV and carcinogenesis, as HBV integration is a risk factor that has been associated with HBV chronicity and hepatocellular carcinoma
