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Human AKT1 (K39R) BAF3 Cell Line

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Home | Transfected Stable Cell Lines | Overexpression Stable Cell Lines | Human AKT1 (K39R) BAF3 Cell Line
Species

Human
Cat.No

ABC-X0018C
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Lymphocyte
Shipping Info

Dry Ice
Growth Conditions

37 °C, 5% CO₂
Source Organ

Lymphatic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Overexpression Stable Cell Lines
Host Cell

BAF3

Description

Human AKT1 (K39R) BAF3 Cell Line is a genetically engineered model derived from selected murine Ba/F3 parental cell line based on customers’ requirement. AKT1 (K39R) BAF3 mutant cell line is generated by stable integration of exogenous human AKT1 gene harboring the K39R point mutation into Ba/F3 host cells using optimized transduction of lentiviral vectors.
Target
AKT1 is a serine/threonine kinase central to cell survival, growth, and metabolism through the PI3K/AKT/mTOR pathway. The K39R mutation, located within the pleckstrin homology (PH) domain, is a kinase-inactivating substitution used to investigate dominant-negative AKT signaling. It serves as a useful tool to dissect the functional relevance of AKT1 activity in growth factor-mediated pathways. AcceGen offers generation of stable overexpression and kinase-dead mutant cell lines targeting any gene of your interest. Polyclonal or monoclonal is optional based on customers’ research needs.

AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • For research use only

  • The AKT1 (K39R) BAF3 Cell Line serves as a powerful tool to investigate AKT1 loss-of-function mechanisms and pathway modulation. This model enables mechanistic studies of PI3K/AKT signaling blockade, IL-3 dependence, and drug response under kinase-inhibited conditions. It is utilized for functional screening of PI3K pathway inhibitors and negative regulatory elements in oncogenic signaling.

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