| Species |
Human
|
| Cat.No |
ABC-KH040Y
|
| Quality Control |
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
|
| Product Category |
Transfected Stable Cell Lines |
| Size/Quantity |
1 vial
|
| Cell Type |
Epithelial
|
| Shipping Info |
Dry Ice
|
| Growth Conditions |
37 ℃, 5% CO2
|
| Source Organ |
Skin
|
| Disease |
Epidermoid Cancer
|
| Biosafety Level |
1
|
| Storage |
Liquid Nitrogen
|
| Product Type |
Knockout Stable Cell Line
|
| Host Cell |
A-431
|
| Gene Info |
B2M
|
Description
Human B2M Knockout A-431 Cell Line is generated from human epidermoid carcinoma A-431 cells through CRISPR/Cas9-mediated disruption of the Beta-2-Microglobulin (B2M) gene, an essential component of MHC class I molecules required for antigen presentation. This knockout model exhibits complete loss of surface MHC class I expression while maintaining A-431 cells’ characteristic epithelial morphology, making it particularly valuable for studying immune evasion mechanisms in cancer immunotherapy. The cell line serves as a critical tool for research involving NK cell-mediated cytotoxicity, T-cell recognition, and immune checkpoint resistance, with specific applications in developing universal CAR-T cell therapies. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through multiplex analysis including genomic PCR, Sanger sequencing, and flow cytometry confirming absence of MHC class I surface expression. The cell line has undergone rigorous quality control and is certified free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.
