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Transfected Stable Cell Lines

Human BRAF (G464V) BAF3 Cell Line

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Home | Transfected Stable Cell Lines | Overexpression Stable Cell Lines | Human BRAF (G464V) BAF3 Cell Line
Species

Human
Cat.No

ABC-X0295C
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Lymphocytes
Shipping Info

Dry Ice
Growth Conditions

37 °C, 5% CO2
Source Organ

Lymphatic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Overexpression Stable Cell Lines
Host Cell

BAF3

Description

Human BRAF (G464V) BAF3 Cell Line is derived from Ba/F3 cells through stable lentiviral integration of human BRAF carrying the G464V mutation. Expanded below passage 20, gene overexpression is confirmed by qRT-PCR.
Target
The G464V mutation enhances BRAF kinase activity and occurs in a subset of solid tumors, such as colorectal and thyroid cancer. It is associated with RAF dimerization and MEK-ERK hyperactivation. AcceGen offers generation of stable overexpression cell lines targeting any gene of your interest. Polyclonal or monoclonal is optional based on customers’ research needs.

AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • For research use only

  • This model enables drug screening against non-V600 BRAF mutants and supports mechanistic studies of RAF dimer-targeted strategies.

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