Human Cardiac Fibroblasts-Ventricular

Description

Human Cardiac Fibroblasts-Ventricular (HCF-v) are derived from human ventricular tissue and cryopreserved at early passage. HCF-v exhibit a typical spindle-shaped morphology and adherent growth in monolayers under standard culture conditions. These fibroblasts are one of the most abundant cell types in the heart, providing structural support to cardiomyocytes and playing a central role in cardiac development and pathological remodeling. These primary human heart ventricular fibroblasts synthesize extracellular matrix components and secrete paracrine growth factors, contributing to scar formation following myocardial infarction and cardiac fibrosis. They are widely used as an in vitro model to study cardiac matrix remodeling induced by physiological (e.g., exercise) and pathological (e.g., hypertension) stressors. HCF-v are identified by immunofluorescence using antibodies against fibronectin. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, syphilis, mycoplasma, fungi, yeast, and bacteria. Repeated freeze-thaw cycles should be avoided during handling and culture.Human Cardiac Fibroblasts-Ventricular (HCF-v) are derived from human ventricular tissue and cryopreserved at early passage. HCF-v exhibit a typical spindle-shaped morphology and adherent growth in monolayers under standard culture conditions. These fibroblasts are one of the most abundant cell types in the heart, providing structural support to cardiomyocytes and playing a central role in cardiac development and pathological remodeling. These primary human heart ventricular fibroblasts synthesize extracellular matrix components and secrete paracrine growth factors, contributing to scar formation following myocardial infarction and cardiac fibrosis. They are widely used as an in vitro model to study cardiac matrix remodeling induced by physiological (e.g., exercise) and pathological (e.g., hypertension) stressors. HCF-v are identified by immunofluorescence using antibodies against fibronectin. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, syphilis, mycoplasma, fungi, yeast, and bacteria. Repeated freeze-thaw cycles should be avoided during handling and culture.