Human Chronic Lymphoid Leukemia Peripheral Blood Mononuclear Cells (Relapsed/Refractory)

Description

Human Chronic Lymphocytic Leukemia Peripheral Blood Mononuclear Cells (Relapsed/Refractory) are derived from the peripheral blood of patients with relapsed or refractory chronic lymphocytic leukemia (CLL).Morphologically, the cells are mostly typical small lymphocyte-like, with small and round cell bodies, round or slightly irregular nuclei, and highly condensed chromatin. Relapsed/refractory CLL cells often exhibit cytogenetic abnormalities such as del(17p), del(11q), or trisomy 12, detectable by FISH or conventional karyotyping.When cultured in vitro, the cells grow in suspension. They commonly express CD19, CD5, CD23, and show aberrant expression of BCL2 and resistance-associated markers such as TP53 mutations. Detection is typically done using flow cytometry and PCR sequencing.These cells are directly related to relapsed/refractory CLL, and their clonal proliferation and abnormal survival lead to disease progression. Patients often experience symptoms such as anemia, infection, increased risk of bleeding, and organ involvement.