Human Chronic Myeloid Leukemia Peripheral Blood Mononuclear Cells (Accelerated/Blast Crisis)

Description

Human Chronic Myeloid Leukemia Peripheral Blood Mononuclear Cells (Accelerated/Blast Crisis) are mononuclear cell populations isolated from the peripheral blood of patients with chronic myeloid leukemia (CML) in the accelerated phase or blast phase. Morphologically, cells in the accelerated phase show abnormal differentiation of granulocytes and mononuclear cells, and imbalance of nuclear cytoplasm ratio.CML-AP/BC cells typically exhibit the Philadelphia chromosome (t(9;22)(q34;q11)), along with secondary chromosomal abnormalities like trisomy 8 or i(17q), detected via karyotyping or FISH. In the blast phase, blasts are the main cells, with reduced cytoplasmic granules, loose nuclear chromatin, and prominent nucleoli.These cells highly express CD34, CD117, and BCR-ABL1 fusion transcripts, detected by flow cytometry and RT-PCR. Its functional abnormality is manifested by the continuous activation of tyrosine kinase driven by the BCR-ABL1 fusion gene, which regulates the cell cycle through signaling pathways such as Ras and PI3K, inhibits apoptosis and promotes proliferation.

Application

• Human Chronic Myeloid Leukemia Peripheral Blood Mononuclear Cells (Accelerated/Blast Crisis) can serve as an ideal model for studying BCR-ABL1 oncogenic signaling. By analyzing its abnormally activated Ras/MAPK, PI3K/AKT and other pathways, the molecular mechanism of the transformation of CML from the chronic phase to the progressive phase is revealed.