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Primary Cells

Human Colonic Epithelial Cells

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The colorectum is a major organ for both malignant and nonmalignant diseases. Cells that line the colonic mucosal surface form a major mechanical barrier that separates the host’s internal milieu from the external environment. In addition to the well-established role of epithelial cells in ion transport, these cells appear to function as an integral component […]
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Home | Primary Cells | Human Primary Cells | Digestive System Primary Cells | Human Colonic Epithelial Cells
Product Code

HCoEpiC
Species

Human
Cat.No

ABC-TC3570
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Epithelial
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Colonic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Intestinal Cells
Key Features

-Backed by AcceGen advanced technology
-Cryopreserved for highest viability and plating efficiency
-Quality-tested for accurate results
-Expert teams to provide timely support and assist best experimental outcomes

Description

Human Colonic Epithelial Cells are isolated from human colon tissue. In vitro, the cells grow adherently, exhibit typical epithelial morphology and are closely arranged to form a barrier structure. and are closely arranged to form a barrier structure. Primary human colonic epithelial cells are usually provided at early passages (passage 1–3).Their functions include absorbing nutrients, secreting mucus to lubricate the intestines, transporting waste, and participating in mucosal immune responses, and can identify and eliminate harmful microorganisms and antigens. When the colon is damaged, the cells have the ability to regenerate and can proliferate and differentiate into new epithelial cells to participate in tissue repair.They express epithelial markers such as cytokeratin 20 (CK20) and E-cadherin, which can be detected by immunostaining.

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Citation

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Application

  • Human Colonic Epithelial Cells can be used to explore the physiological functions of the colon, such as nutrient absorption, mucus secretion, and immune regulation mechanisms, and help to understand the interaction between the intestinal microbiota and the host. These cells can also be used to study inflammatory bowel diseases, colon cancer, and irritable bowel syndrome.

Frequently Asked Questions

  • What are Human Colonic Epithelial Cells?

    Human Colonic Epithelial Cells are cells that line the inner surface of the colon. They are essential for absorbing water and electrolytes, producing mucus, and forming a barrier against pathogens and toxins.

  • How many times can Human Colonic Epithelial Cells be passaged?

    Human Colonic Epithelial Cells can typically be passaged 3-5 times. However, with each passage, their epithelial characteristics may weaken. Therefore, it is generally recommended to use these cells within three passages to ensure their functionality and reliability in experiments.

  • What are the differences between Human Colonic Epithelial Cells and human intestinal epithelial cells?

    Functionally, Human Colonic Epithelial Cells are specialized for absorbing water and electrolytes, producing mucus, and maintaining colonic health, whereas human intestinal epithelial cells focus more on nutrient absorption and interaction with gut microbiota.

  • How are Human Colonic Epithelial Cells used in drug development and testing?

    Human Colonic Epithelial Cells are utilized in drug development to study drug absorption, metabolism, and toxicity specific to the colonic environment. They help researchers understand how drugs interact with colonic epithelial cells and assess their effectiveness and safety in treating colonic diseases such as ulcerative colitis and colorectal cancer.

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