Human Cystic Fibrosis Peripheral Blood Mononuclear Cells

Description

Human Cystic Fibrosis Peripheral Blood Mononuclear Cells (PBMCs) are isolated from the peripheral blood of patients with cystic fibrosis (CF), mainly including lymphocytes, monocytes and dendritic cells. Morphologically, PBMCs are round or oval. PBMCs from CF patients play a key role in immune regulation and inflammatory response. Due to the mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, the chloride channel function is defective, which causes abnormal extracellular environment and thus affects the function of PBMCs. These cells show excessive inflammatory response, such as increased secretion of proinflammatory cytokines (TNF-α, IL-6, IL-8), and increased oxidative stress levels, which aggravate chronic lung infection and tissue damage.