Human Dermal Fibroblasts, Adult

Description

Human Dermal Fibroblasts, Adult are primary cells isolated from the dermal layer of human skin. These cells exhibit an adherent culture properties, forming confluent monolayers with a dense, overlapping growth pattern. Functionally, HDFa synthesize extracellular matrix components like collagen and elastin, orchestrate wound healing through fibronectin-mediated migration, and interact with epidermal cells during tissue repair and hair follicle development. Human Dermal Fibroblasts demonstrate mesenchymal stem cell-like properties, expressing surface markers CD73, CD90, and CD105. They serve as cellular substrates for induced pluripotent stem cell (iPSC) reprogramming using the Yamanaka transcription factors (Oct3/4, Sox2, Klf4, c-Myc).

Application

• HDFa serve as a versatile model system for investigating various facets of cell physiology and have been prominently featured in numerous research works, especially within the realms of skin biology and reprogramming studies. These fibroblasts find application in diverse areas such as pathogen response, skin aging, wound healing, gene delivery, and skin-related disorders like scleroderma. Their utility extends to research involving ECM protein analysis, collagen metabolism, cosmetics, skin therapy, and model development, encompassing an array of multidisciplinary research avenues.