Human FGFR3 (S249C_V555M) NIH/3T3 Cell Line

Description

Human FGFR3 (S249C_V555M) NIH/3T3 Overexpression Cell Line is a genetically engineered model derived from selected murine NIH/3T3 fibroblast parental cells based on customers’ requirement. FGFR3 (S249C_V555M) NIH/3T3 overexpression cell line is generated by stable integration of exogenous human FGFR3 double mutant (S249C and V555M) into NIH/3T3 host cells using our optimized transduction of lentiviral vectors. The expression cassette contains the full-length FGFR3 open reading frame carrying both activating mutations. The edited cells are maintained at low passage numbers (<P20). Overexpression clone is validated at gene level by qRT-PCR. Target FGFR3 (Fibroblast Growth Factor Receptor 3) is a receptor tyrosine kinase involved in cell growth, differentiation, and angiogenesis. Mutations like S249C and V555M are recurrent in cancers such as bladder cancer and multiple myeloma, and lead to ligand-independent activation and downstream oncogenic signaling. AcceGen offers generation of stable overexpression cell lines targeting any gene of your interest. Polyclonal or monoclonal is optional based on customers’ research needs.